non effective 29 mer scrambled negative control shrna  (OriGene)

Journal: Cell reports

Article Title: Pbrm1 Steers Mesenchymal Stromal Cell Osteolineage Differentiation by Integrating PBAF-Dependent Chromatin Remodeling and BMP/TGF-β Signaling.

doi: 10.1016/j.celrep.2020.107570

Figure Lengend Snippet: Figure 2. Loss of Pbrm1 and PBAFs Impairs MSC Osteogeneic Potential (A) Immunoblot analysis showing expression of PBAFs in OP9 cells stably expressing sh-Pbrm1 (sh-Pbrm1_1 and sh-Pbrm1_2 represent two different shRNA expressing constructs) or sg-Arid2/cas9 or sg-Brd7/Cas9. (B) qRT-PCR (normalized to 0 h) of Alpl (left) and Osx (right) in control or Pbrm1-deficient OP9 cells treated with BMP-2. (C) Alkaline phosphatase staining of control or Pbrm1-deficient OP9 cells differentiated with osteogenic conditioned media over 21 days. (D) qRT-PCR (normalized to 0 days) of Alpl (left) and Osx (right) in control or Pbrm1-deficient OP9 cells after 21 days of osteogenic differentiation. (E) qRT-PCR (normalized to 0 h) of ALPL (left) and OSX (right) in control or PBRM1-deficient HS-5 cells treated with BMP-4/7.

Article Snippet: Lentiviral plasmid constructs expressing sh-Pbrm1_B (TL517996B), sh-Pbrm1_C (TL517996C) and scrambled negative control non-effective shRNA cassette sh-Control (TR30021) were purchased from Origene.

Techniques: Western Blot, Expressing, Stable Transfection, shRNA, Construct, Quantitative RT-PCR, Control, Staining

Journal: Cell stem cell

Article Title: TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment

doi: 10.1016/j.stem.2018.12.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Scrambled negative control non-effective shRNA , Origene , Cat #TR30021.

Techniques: Purification, Recombinant, Knock-Out, Blocking Assay, Plasmid Preparation, Cloning, One Step RT-PCR, SYBR Green Assay, Magnetic Beads, Staining, Sequencing, CRISPR, Negative Control, shRNA, Expressing, Software